Aequorin photoprotein test
The photoprotein aequorin has been widely used as a bioluminescent label in immunoassays, for the determination of calcium concentrations in vivo, and as a reporter in cellular imaging. It is composed of apoaequorin (189 amino acid residues), the imidazopyrazine chromophore coelenterazine and molecular oxygen.
As of 2014, we have not seen any research with the use of aequorin as a supplement in humans. A form of aequorin is claimed to be found in Prevagen, a jelly fish extract.
The recombinant calcium sensitive photoprotein aequorin was the first probe used to measure specifically the Ca2+ concentration, [Ca2+], inside the intracellular organelles of intact cells.
Use of aequorin for G protein-coupled receptor hit
identification and compound profiling.
Methods Mol Biol. 2009; Brough SJ. Department of Screening and Compound Profiling, GlaxoSmithKline, Essex, UK.
G protein-coupled receptors (GPCRs) represent the largest class of targets in drug discovery, one-third of all marketed drugs are active at GPCRs and drugs targeted at GPCRs are marketed in virtually every therapeutic area. GPCRs can be classified by virtue of their coupling to second messenger signaling systems. In the last decade functional evaluation of Galphaq-coupled GPCRs has been enabled by advances in fluorescence dye-based methodologies and detection instrumentation. Investigations into the bioluminescence of jelly fish in the early 1960s isolated the photoprotein aequorin that required only the addition of calcium to generate a luminescent signal. The recent development of sensitive detection platforms with integrated fluidics for liquid handling has revived interest in bioluminescence as an alternative to chemical fluorophore-based detection for characterizing the pharmacology of this target class. In this chapter we describe a detailed methodology for the development and execution of bioluminescence apoprotein aequorin-based screens for hit identification and structure-activity relationship compound profiling and highlight the opportunities and challenges associated with this technique.
Spectral components of bioluminescence of aequorin and
J Photochem Photobiol B. 2008:1 Belogurova NV, Kudryasheva NS, Alieva RR, Sizykh AG. Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russia; Siberian Federal University, Krasnoyarsk, Russia.
Complex bioluminescence spectra of photoproteins from marine coelenterates - jellyfish Aequorea victoria and hydroid Obelia longissima, and photoluminescence spectra of the bioluminescent reaction products (Ca(2+)-discharged photoproteins) were deconvolved into components. The bioluminescence spectra of aequorin were found to include three, the bioluminescence spectra of obelin - four, and the photoluminescence spectra of the Ca(2+)-discharged photoproteins - only two components.
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